IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Crystallization and Preliminary x-ray Analysis of Nucleoside Diphosphate Kinase 1 from T. cruzi.
Autor/es:
GÓMEZ BARROSO JA, MIRANDA MR, PEREIRA CA, AGUILAR CF
Lugar:
Tucumán, Argentina
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2009
Institución organizadora:
Sociedad de Investigación en Bioquímica y Biología Molecular
Resumen:
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Trypanosoma cruzi is the etiologic agent of Chagas' disease. The Nucleoside diphosphate
kinases (NDPKs) are enzymes involved in energy management and nucleoside
balance in the cell. T. cruzi
TcNDPK1, a canonical isoform. The objective of this work is obtaining proteins
crystals, diffract and process the data for tridimensional structure
resolution. Materials and Methods: TcNDPK1 was expressed in E. coli as a
fusion protein with N-terminal His-tag. TcNDPK1 was overexpressed and purified
by FPLC. Crystallization was assayed by sitting drop and hanging drop vapor
diffusion method. Crystals was frozen and
diffracted on synchotron x-ray radiation in Campinas (Brasil). The data set collected was
reduced and merged using MOSFLM and SCALA programs. Results and Discussion:
His-TcNDPK was overexpressed, purified and crystallized. The crystals are diffracted
and collected the data to 3.5Å. The crystals belong to the trigonal space group
P3, with unit cell parameters a=127.94, b=127.84, c=275.49. Structure
determination is under way. These results will provide relevant information
that could be the first step in rational drug design for treating Chagas disease.