PACHYPODANTHINE: AN APORPHINOID ALKALOID THAT INHIBIT PLANKTONIC GROWTH OF YERSINIA ENTEROCOLITICA

MASTRODONATO ANNA CHIARA; PUNGITORE CARLOS; DI MARCO NATALIA; LUCERO ESTRADA, CECILIA S. M.

SAN LUIS

Congreso; XIII Congreso Argentino de Microbiología General SAMIGE 2018; 2018

Yersinia enterocolitica is a Gram-negative coccobacillus belonging to Enterobactereaceae family. It isan important foodborne pathogen causing gastrointestinal disease in humans known as yersiniosis. Y.enterocolitica is widespread in the environment and animal populations. The main reservoir of humanpathogenic Y. enterocolitica strains are pigs. So, contaminated pork and pork products are the mostimportant source of infection. Due to the inappropriate use of antibiotics in the last years has allowedmicroorganisms evolve resistance to them, it is important to look for an alternative to battle againstbacteria. Alkaloids are abundant secondary metabolites in plants and represent one of the mostwidespread classes of compounds endowed with multiple and varied pharmacological properties. Withthe intention of contributing to solve this global problem, in previous studies, we examined a set ofaporphinoid alkaloids as possible effective antimicrobial agents on planktonic growth of Y.enterocolitica strain. We found that Pachypodanthine inhibited planktonic growth at 100 µM. The aimsof this study are determinate the Minimal Inhibitory Concentration (MIC) and Minimal BactericidalConcentration (MBC) and evaluate the growth inhibitory effect at 96 h of culture of Pachypodanthineon Y. enterocolitica bio-serotype B1A/O:7,8-8-8,19, isolated from pork sausage in our laboratory. TheMIC and MBC was determined by the microdilution test in Moeller Hinton broth (MH) according toClinical and Laboratory Standards Institute (CLSI), with 100 µM of initial concentration and subsequentserial dilutions in half, at 37 °C for 24 h. A growth curve was made with an starter inoculum at DO 610of 0.05 in MH broth using the inhibitor at MIC concentration. Aliquots of 0.1 ml were measured atOD655 after 3, 6, 18, 24, 48, 72 and 96 h of culture at 37°C using a microplate reader. Results showthat MIC value was 100 µM, as this was the highest tested concentration it was not possible to obtainthe MCB value. Its inhibition increased from 62.5 % at 24 h to 76.9 % at 96 h of culture with MIC value.In addition, we assayed MIC and MBC on different biotypes (B) of Y. enterocolitica strains, resultingfor B1B strain 100 µM; for B2 and B3 strains 50 µM; and for B4 25 µM for both determinations in allcases. These results suggest that Pachypodanthine presents great inhibition of Y. enterocoliticaB1Aplanktonic growth at 100 µM improving its effect until 96 h of culture, indicating that it does notdegrade over time. Furthermore, this compound can inhibit planktonic growth of different Y.enterocolitica biotypes showing activity in almost all existing biotypes. We concluded thatPachypodanthine could be used as antimicrobial agent in the future to prevent intestinal diseases.