Renin - angiotensin system polymorphisms association with obesity and some risk factors in hypertension patients

ARCE ME; CORREA MM; FUENTES LB

Mendoza

Congreso; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2018

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Hypertension (HTA) is the clinical expression of genetic, physiological, and biochemical interaction to maintain cardiovascular homeostasis. The renin-angiotensin system (RAS) has an important role in the regulation of blood pressure. RAS polymorphisms have been studied as candidate risk factors for hypertension. The study of polymorphism is very controversial, and its identification in HTA patients is required. The aim was to investigate a possible involvement of angiotensin converting enzyme insertion/deletion (ACE I/D) and angiotensin II receptor 1 (AT1) A1166C polymorphisms association with obesity and some clinical variables related to HTA. A total of 230 hypertensive patients and 167 controls subjects were recruited. Blood pressure and body measurements were recorded and a fasting blood sample was obtained for the quantitation of glucose, lipid profile, uric acid and identification of polymorphisms. Subjects were grouped based on hypertension and excess body weight (overweight and obesity) based to WHO classification. ACE I/D polymorphisms were identified by polymerase chain reaction (PCR) and AT1 A1166C was performed using PCR-RFLP (restriction fragment length polymorphism) methods. The BMI>25.0 kg/m2 in hypertensive patients represented 87.4% (52.7% men, 47.3% women) and normotensive 63.9% (37.2% men, 62.8% women). The allele and genotype frequency of A1166C polymorphism showed no significant difference between HTA and controls. The genotypes CC in studied population (BMI>25.0 kg/m2) was statistically significant compared to AA (p