EFFECTS OF DIFFERENT NUTRITIONAL DIETS ON RAT MAMMARY GLAND RESPONSE TO CADMIUM ENVIRONMENTAL INTOXICATION

MICHEL MC; ALZA N; PEREZ CHACA MV; ALVAREZ SM; SANCHEZ ES; ARNAL N; MARTÍN MOLINERO GD; GIMENEZ MS; VASQUEZ E; BOLDRINI G; GOMEZ N

Mar del Plata

Congreso; Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2018

SAIC

Cadmium (Cd) is a toxic element and an important environmentalcontaminant. We studied the effects of this metal at the molecular,biochemical and cellular levels on rat mammary gland (MG) andlooked at the potential protective outcome of a Soy based diet. Forthis purpose, 4 female lots of Wistar rats were used: 2 lots receivedcasein and 2 lots soybean (Soy) as protein source. Within eachgroup, 1 lot received regular water (control) and the other, 15 ppmof Cd in the drinking water for 60 days (n= 6 animals per group,from three independent experiments). Lipids were extracted by Hexano:Isopropanol:BHT (3v:2v:1%). Total cholesterol, phospholipidsand fatty acids were determined by colorimetric assay, thin-layerchromatography and gas chromatography-mass spectrometry, respectively.HMG CoA Reductase (HMGCoAR) and NFκB activationwere determined by Western blot. The inflammation marker COX2and the Bax/Bcl2 ratio were measured by RT-PCR. MGs were subjectedto hematoxylin-eosin stain for morphological studies. Our resultsshow that Cd alters the lipid profile of the gland and this effectis modulated by Soy. The expression of HMGCoAR is affected by Cdand Soy (*p