IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Aging Abolishes Circadian Rhythms of RORa and Antioxidant Enzymes Expression in the Temporal Cortex of Free Running Rats"
Autor/es:
NAVIGATORE FONZO LS; ANZULOVICH AC; DEYURKA NA; LACOSTE MG; CORIA-LUCERO C
Lugar:
Mendoza Capital
Reunión:
Congreso; XXXVI Reunion Anual de la Sociedad de Biología del Cuyo; 2018
Institución organizadora:
Sociedad de Biología del Cuyo
Resumen:
The retinoid-related orphan receptor α (RORα) is atranscription factor that binds RORE motifs in the promoter ofclock Bmal 1 and othcr targct genes. Antioxidant enzymes contributeto thc cellular redox statc which is crucial for the molecular clock fuuction. Previously, we showed that antioxidant enzymesactivity follows a daily variation in the temporal cortex(TC), which was abolished in aged rats. Herein our objectives were: 1) to investigate endogenous rhythms of catalase (Cat), glutathioneperoxidase (Gpx) and Nrf2 genes expressicn as well as RORαprotein levels in the rat TC, and 2) to evaluate the agingconsequences on those temporal patterns. Tree- and 22-month-old maleHoltzrnan rats were maintained under constant darkness for 15 days beforethe experiment, in order to validate the endogenous nature of circadianrhythms. On the experiment day, TC samples were isolatedevery 4 h during a 24h period. RORα protein levels were assessed by inununoblotting. Cat, Gpx and Nrf2 mRNA Ievels were dctermined by RT-PCR. Specificsoftware was used to analyze circadian rhythms. We observedcircadian endogenous rhythrns of RORα, Cat and Gpx expressionin the TC of free running young rats (Chronos fit, p