Modulation of glucocorticoid receptor activity by histamine H2 receptor signaling. Involvement of PI3K and mTOR.

ZAPPIA, CARLOS DANIEL; SHAYO, CARINA; GRANJA GALEANO, GINA; FERNANDEZ, NATALIA; TORRALBA-AGU, VALERIA; DAVIO, CARLOS; MONCZOR, FEDERICO

Mar del Plata

Congreso; LXIII Reunión anual de la SAIC; 2018

SAIC

There are reports describing the interaction between membrane G-protein coupled receptors (GPCRs) signaling and glucocorticoid receptor (GR) transcriptional activity. The aim of the present work was to study the modulation of GR activity by histamine H2 receptor (H2r) signaling. HEK293T cells were co-transfected with plasmids coding for H2r, GR and a GR-driven reporter gene TAT3-Luc. In this system, 10 minutes pretreatment with 10μM H2r agonist (amthamine) significantly increased GR activity, duplicating dexamethasone-induced signal (p