CADMIUM ALTERS LEUKOCYTE POPULATION, NITRITES CONCENTRATION AND APOPTOSIS AND INFLAMMATION MARKERS IN LUNG. EFFECT OF DIFFERENT DIETS

BOLDRINI GG; BIAGGIO VS; MARTIN MOLINERO GD; GIMENEZ MS; ALVAREZ SM; GOMEZ NN

Merlo, San Luis

Congreso; Reunión Anual de la Sociedad De Biología de Cuyo; 2017

Sociedad de Biología de Cuyo

Cadmium (Cd) is a toxic metal and an important environmental contaminant. We studied its effects on inflammation and apoptosis markers, leucocytes in bronchoalveolar lavages (BAL) and nitrites concentration under different diets. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein sources. Within each group, 1 lot received regular water (control-Co) and the other, 15ppm of Cd in the drinking water for 60days. BAL was performed, nitrites concentration was measured and cells obtained were counted. Total RNA was isolated with Trizol and cDNA was obtained. Cyclooxygenase-2 (COX-2), transforming growth factor beta (TGF-β), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), vascular cell adhesion molecule-1 (VCAM-1), p53, BAX and Bcl-2 were determined by PCR. S28 was used as control. Nitrites decreased in Cas-Cd and So-Co vs Cas-Co (p