IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cadmium chronic intoxication: modulation of oxidative stress in the rat heart by the diet.
Autor/es:
FERRAMOLA, M.; ANZULOVICH AC; GIMÉNEZ MS
Lugar:
San Miguel deTucuman, Argentina
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2009
Resumen:
The aim of this study was to assess the effect of Cd2+ on oxidative
stress parameters in heart, and the putative protection of a soy-based
diet. Two months old male Wistar rats were separated into six
groups: 1, 3 and 5 were fed with a casein-based diet; 2, 4 and 6 were
fed with a soy-based diet. Controls (1 and 2) received tap water;
groups 3 and 4 received tap water with Cd2+ 15ppm; groups 5 and 6,
tap water with Cd2+ 100ppm. Food and water were available ad
libitum, during 4 months. TBARS levels and paraoxonase-1 (PON-
1) activity in serum, and antioxidant enzymes activities (SOD, CAT
and GPx) in heart were determined spectrophotometrically. TBARS
levels and PON-1 activity increased in group3, compared to 1,
however TBARS decreased in group 6, compared to 2 (p<0.05).
CAT activity increased in group 3 in comparison to 1, and it
decreased in groups 4 and 6, compared to 2 (p<0.05). No differences
were found in GPx or SOD activities.
In this study, we observed that Cd2+ intoxication increases oxidative
stress in serum and heart, modifying at the same time antioxidant
enzymes activities. There seems to be an induction of CAT activity
in the heart and PON-1 activity in serum with the lower
concentration of the metal. This, probably, could be due to a defense
mechanism. No increases of lipid peroxidation in serum were
observed in the rats fed with the soy-based diet.