Temporal study of DNA methyltransferases, BDNF and TrkB in hippocampus and cerebellum during aging

ALTAMIRANO FG; PONCE IT; CASTRO-PASCUAL IC; DELGADO SM; FERRAMOLA ML; ANZULOVICH AC; CARGNELUTTI E; LACOSTE MG

Ciudad Autónoma de Buenos Aires

Congreso; II FALAN Congress; 2016

Federation of Latin-American and Caribbean Societies for Neuroscience

Epigenetic mechanisms, including DNA methylation which plays a key role in memory and motor learning, are altered by aging. We aimed to evaluate the circadian variation of DNA methyltransferases (Dnmts), Bdnf and TrkB expression in young and aged rats. Holtzman 3- and 22-month old male rats were maintained in constant darkness conditions during 15 days. Bdnf, TrkB, Dnmt1, Dnmt3a and Dnmt3b mRNA levels were determined in hippocampus and cerebellum, isolated at 4h-intervals. We previously observed that Bdnf and TrkB mRNA expression oscillated in a circadian way (p