PRENATAL ACE INHIBITORS MODIFY THE IMMUNOFLUORESCENCE OF ANGIOTENSIN II RECEPTORS DURING POSTNATAL LUNG DEVELOPMENT

ROCIO A COMFORTI; LUCIA B. FUENTES.; SUSANA I SANCHEZ; FLORENCIA M SOLER GARCÍA; GLADYS M CIUFFO

Mar del Plata

Congreso; Reunión Conjunta Sociedad Argentina de Investigación Clínica (SAIC), Sociedad Argentina de Inmunología (SAI) y Sociedad Argentina de Farmacología Experimental. Mar del Plata, noviembre 2016.; 2016

Reunión Conjunta Sociedad Argentina de Investigación Clínica (SAIC), Sociedad Argentina de Inmunología (SAI) y Sociedad Argentina de Farmacología Experimental. Mar del Plata, noviembre 2016.

The renin?angiotensin system (RAS) is a classical endocrine system regulating blood pressure, electrolyte and fluid homeostasis. The activity and distribution of RAS are developmentally regulated in a tissue-specific manner, and angiotensin II (Ang II) can act as a modulator of growth in a variety of cells and tissues. Different components of the RAS, including angiotensin-converting enzyme (ACE) and both angiotensin type 1 (AT1) and type 2 (AT2) receptors, are expressed in lung and several evidences from the literature demonstrated a possible involvement during lung development. However, RAS components expression pattern, during posnatal lung morphogenesis, is largely unknown. ACE inhibitors are among the most widely prescribed antihypertensive agents, but when used in the second half of pregnancy, they can cause several abnormalities. The aim of this study was to test the effects of prenatal ACE inhibition on the presence and subtype distribution of angiotensin II receptors postnatal lung development. Pregnant Wistar rats (230-250g) on the 13th day of pregnancy were randomly assigned to three experimental groups: control (n=6), Captopril (n=6) and Enalapril treated (n=6) rats. Mini-osmotic pumps were implanted subcutaneously, containing sterile isotonic saline solution (control), Enalapril or Captopril in a dose 2,85 mg/kg/day. The in vivo effects of prenatal exposure ACE inhibitors were evaluated at different postnatal ages: 0 (P0), 8 (P8) and 15 (P15) on the presence and localization of Ang II receptors by immunofluorescence. AT1 receptors were localized in the stroma underlying the epithelium of bronchioles and vascular smooth muscle cells in control y treated animals at the different ages studied. The AT1 levels were modify in bronchioles, Enalapril treatment produced a significant decrease at P0 (p