Dietary soy protein improves adipose tissue dysfunction by modulating parameters associated with oxidative stress in rats fed a sucrose-rich diet

DANTE ALEJANDRO SELENSCIG; YOLANDA B. LOMBARDO; PAOLA GUADALUPE ILLESCA; MARIA DEL ROSARIO FERREIRA; MARIA EUGENIA D'ALESSANDRO; SILVINA MONICA ALVAREZ; MARIA SOFIA GIMENEZ

Mar del Plata

Congreso; LXI Reunión de la Sociedad Argentina de Investigación Clínica (SAIC). LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI). XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE). VII Reunión Cinetífica Anual de la S; 2016

Sociedad Argentina de Investigación Clinica

Numerous studies link dietary soy protein with beneficial effects on different metabolic disorders (dyslipidemia, diabetes, atherosclerosis). One of the potential mechanisms mediating these effects might be the reduction of oxidative stress. The adipose tissue is especially susceptible to the damage of oxidative stress. The aim of this study was to evaluate the possible beneficial effect of dietary intake of soy protein isolated (SPI) on adipose tissue (AT) dysfunction (antioxidant defenses, reactive oxygen substances (ROS) production and visceral adiposity) in a rat model that mimics several aspects of the human metabolic Syndrome. Male Wistar rats were fed a sucrose rich diet (SRD) (62.5% sucrose) for 4 months after that were divided into two subgroups, one continued with SRD until month 8, while the other received SRD in which casein was replaced by SPI for an additional 4 months. A reference group consumed a control diet all the time. In AT were determined: i) the activities of antioxidant enzymes (SOD, CAT, GPx), GR and gene expression of Mn-SOD, GPx ii) the activity of xanthine oxidase (XO), ROS levels and the gene expression of NAD(P)H oxidase iii) the expression of Nrf-2 transcription factor. Besides, plasma levels of uric acid, protein carbonyl, TBARS and the inflammatory citoquine TNF-α were also determined. Compared with the SRD-fed rats, SRD-S normalizes the activities of SOD and GR, improves or normalizes gene expression of SOD and GPx respectively without changes in the expression of Nrf2. In addition, both XO activity was normalized and tissue ROS levels improved. The SPI decreased TNF-α and restored plasma levels of uric acid, protein carbonyl group and TBARS. The present study show a beneficial effects of SPIupon dysfunctioned adipose tissue in dyslipemic, insulin resistant rat model, suggesting that soy protein can be a complementary nutrient in the metabolic syndrome.