Cadmium exposition during gestation (20G) and lactancy (PND15) alters antioxidant defense. Study of effect of soy protein as a protein source.

BIAGGIO VS; PEREZ CHACA MV; ALTAMIRANO KN; DELLA VEDOVA MC; PIGUILLEM SN; GIMENEZ, MS

Mar del Plata

Congreso; SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLINICA Y BIOQUIMICA; 2016

Introduction: Exposure to toxic metals during pregnancy is one factor that alters the fetal environment and consequently increases the risk of metabolic disorders in adulthood. Cadmium (Cd) accumulates in the placenta inducing low birth weight and oxidative stress. However, little is known about the effect of soy protein during pregnancy and lactation. Our objective was to evaluate the possible protective role of soy protein consumption versus Cd mechanisms by which exerts its toxicity.Methods: we worked with 4 lots of female Wistar rats; 2 lots received casein and two lots soy, as a protein source. 1 lot in each group received tap water and the other 15ppm of Cd in drinking water during pregnancy (20G) and 15 days after birth (PND15). We determined Cd concentration and TBARS, nitrites, carbonyls, proteins and total antioxidant capacity (TAC) levels. Brain tissue total RNA was extracted and RT-PCR was performed using the following primers: MT I; MT II; MT III; Nrf-2; NOX-2, SOD and GPx-1. Sections of brain tissue were performed to histology study. Results: at 20G and PND15 Cd concentration increased in both intoxicated groups (p