CADMIUM ALTERS MORPHOLOGY, APOPTOSIS AND INFLAMMATION MARKERS IN LUNG. EFFECT OF DIFFERENT DIETS

BOLDRINI GABRIEL GIEZI; BIAGGIO V; ALVAREZ SILVINA M; GOMEZ NIDIA N; MARTIN MOLINERO GLENDA D; GIMENEZ MARIA SOFIA

Mar del Plata

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigacion Clinica,; 2016

SAIC

Cadmium (Cd) is a toxic metal and an important environmental contaminant. We studied its effects on apoptosis and inflammation markers, leucocytes proportion in bronchoalveolar lavages and the histoarchitecture of rat lung under different diets. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein sources. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water for 60 days. Bronchoalveolar lavage was performed and cells obtained were counted. Lungs were fixed, sectioned, stained, and examined for evidence of injury. Total RNA was isolated with Trizol and cDNA was obtained. Cyclooxygenase-2 (COX-2), transforming growth factor beta (TGF-β), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), FAS-ligand (FASL), p53, BAX and Bcl-2 were determined by PCR. S28 was used as control. Lymphocytes and neutrophils were augmented in both Cd groups. NF-κB showed a significant decreased in Soy groups vs Cas groups (p