CHRONIC INTOXICATION WITH CADMIUM INDUCES HYPERTENSION TOGETHER WITH OXIDATIVE STRESS, AND APOPTOSIS IN RAT AORTA

BOLDRINI GG; GOMEZ NN; BOLDRINI GG; GOMEZ NN; ALVAREZ SM; PIGUILLEM SN; ALVAREZ SM; PIGUILLEM SN; MARTIN MOLINERO GD; GIMENEZ MS; MARTIN MOLINERO GD; GIMENEZ MS

Mar del Plata

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2016

Sociedad Argentina de Investigación Clínica

Cadmium (Cd) is a toxic metal and an important environmental contaminant. We studied its effects on blood pressure, histoarchitecture,oxidative stress and apoptosis markers of rat aorta. Male Wistar rats were used: 1 group received regular water (control (Co)) and the other, 15 ppm of Cd in the drinking water for 60 days (Cd). During the treatment, blood pressure was measured with a CODA system. Total RNA was isolated with Trizol and cDNA was obtained. Nrf-2 factor, NOX2, p47, GPx, SOD, NF-κB, p53, Transforming growth factor beta (TGF-), VCAM, BAX, Bcl-2 and FAS ligand were determined by PCR. BAX/Bcl-2 ratio was calculated. S28 was used as control. Aortas were fixed, sectioned, stained, and examined for evidence of injury.Cd induced significant increase in systolic and diastolic blood pressure (p