Lipid composition of mononuclear and polymorphonuclear leukocytes in hypothyroid rats

CORIA MJ, GIMENEZ MS

Mendoza

Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2008

Sociedad de Biología de Cuyo

Lipid composition of mononuclear and polymorphonuclear leukocytes in  hypothyroid rats Coria MJ, Gimenez  MS IMIBIO- CONICET- Univ Nac de San Luis E-mail: marie_449@yahoo.com.ar Thyroid hormones are very important regulator of lipid metabolism. Lipid of monocytes (MONO) and polymorphonuclear (PMN) membranes are a major source of mediators of inflammation. The aim of our study was to investigate the influence of hypothyroidism induced by propylthiouracil (PTU) on lipid composition of MONO and PMN leukocytes. Adult Wistar female rats were ramdomly separated in two groups: one received PTU-free drinking water (Control group) while the other received 0.1% PTU in drinking water during 30 days (hypothyroid group, HT). Afterwards the rats were sacrificed and blood samples were taken to determine serum levels of total cholesterol (Col), HDL-cholesterol (HDL-col) and triglycerides (TG). Levels of LDL-cholesterol (LDL-col) were calculated with the Friedwald formula. PMN and MONO were isolated from blood by gradient density to determine them in phospholipids, cholesterol and total lipids. We found the LDL-Col and Col serum levels were increased and TG levels were lower in HT group .The HDL-Col levels did not differ. There were no significant differences in the total lipid content or phospholipids in MONO and PMN, however cholesterol levels in both leukocytes of the HT group were significantly lower. According to our results, the hypothyroidism modifies lipid composition of PMN and MONO leukocytes, could possibly affect any of its functions.