IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
STRUCTURAL BIOLOGY OF T. cruzi ENERGETIC METABOLISM PROTEINS AS POTENTIAL TARGET FOR DRUG DESING
Autor/es:
GÓMEZ BARROSO JA, MIRANDA M, STRADELLA F, BOUVIER LA, CANEPA GE, PEREIRA C, AGUILAR CF.
Lugar:
Mendoza
Reunión:
Congreso; XXVI Annual Scientific Meeting of Cuyo Biology Society; 2008
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
STRUCTURAL BIOLOGY OF T. cruzi ENERGETIC METABOLISM
PROTEINS AS POTENTIAL TARGET FOR DRUG
DESING
Gómez Barroso JA, Miranda M, Stradella F, Bouvier LA, Canepa
GE, Pereira C, Aguilar CF.
Lab de Biol Mol Estructural, Univ Nac de San Luis.
E-mail: jagomez@unsl.edu.ar
Trypanosoma cruzi is the etiologic agent of Chagas disease. The
objective of our work is the resolution by X-ray crystallography of
the three-dimensional structure of T. cruzi proteins involved in energetic
metabolism as a first step for rational drug design based on
the structure. The main objective of the Chagas´ Disease Research
Network is the search for protein targets in metabolic routes absent
in the human host and common in trypanosomatids. The T. cruzi
proteins studied in this work are: TcNDPK (Nucleoside Diphosphate
Kinase), TcTAF9 (TATA Binding Factor 9) and TcAdK1
(Adenilate Kinase 1). They are expressed in E. coli as fusion proteins
with N-terminal His-tag. We have overexpressed, purified and
solubilized these proteins for crystallization and other structural
assays. We have carried out bioinformatic studies. TcNDPK was
studied in different cristalization conditions. TcNDPK crystals were
used by X-ray diffraction assays. TcTAF9 was overexpressed, and
solubilized in adequate quality for crystallization assays and other
structural studies. Cristallization screenings were realized for
TcAdK1. The structural particularities of these proteins showed a high potential for drug design.