EFFECT OF A SOY BASED DIET ON RAT CEREBELLUM EXPOSED TO CADMIUM

MARTIN MOLINERO GLENDA D; PEREZ CHACA MARIA VERONICA; ALVAREZ SILVINA M; GOMEZ NIDIA N; BOLDRINI GABRIEL GIEZI; GIMENEZ MARIA SOFIA

Mendoza

Congreso; Reunion Anual de la Soc de Biologia de Cyo; 2016

Sociedad de Biología de Cuyo

Cadmium (Cd) is an important environmental contaminant. We studied its effects and the protective effect of a soy-based diet in cerebellum. Histoarchitecture, antioxidant enzymes activities, oxidative stress markers, myelination proteins and metallothioneins were determined. Female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (Co) and the other, 15 ppm of Cd for 60 days. NF-E2 related nuclear factor (Nrf-2), SOD 2, CAT, GPx, iNOS, VCAM-1, metallothioneins (MT1 , MT2, MT3), neuregulin (NRG 1), myelin-associated glycoprotein (MAG) and proteolipid protein (PLP) mRNAs were determined by PCR. S28 was used as internal control. Histological studies were performed in H-E stained tissues. CAT and GPx activities were measured in cerebellum homogenates.Nrf-2 decreased in both Cd groups and in Soy Co (p