Angiotensin II AT2 receptors in substantia nigra.

ARCE ME; SÁNCHEZ SI; BRUERA MN; CIUFFO GM

mendoza

Congreso; XXXIV Reunión Anual de la Sociedad de Biología de Cuyo.; 2016

Soc. Biol. Cuyo

The renin-angiotensin system (RAS) has been recognized for its critical role in physiological regulation of arterial pressure, as well as sodium and fluid homeostasis. Angiotensin II (Ang II) is the major effector component of RAS and recognizes two receptors subtypes: AT1 (AT1R) and AT2 receptors (AT2R). Evidence has accumulated aabout a role of the central RAS in the establishment of Parkinson disease and other neurological disorders. AT2R have been implicated in processes occurring during brain development and tissue regeneration. AT2R are widely distributed in fetal tissue, but their expression is dramatically decreased after birth. In adult animals, AT2R expression is restricted to a few organs and limited brain areas. The mesencephalic nucleus Substantia nigra (SN) plays a pivotal role in the control of movement,acting as a major input and output center of the basal ganglia. Conflicting results have been reported concerning the distribution of AT2R in the striatum and the SN of mammals. Data about the distribution of AT2R in animal models of Parkinson disease are limited to a few studies. The aim of this work was to study the localization of AT2R in SN of adult rats. We investigated the expression of AT2R gene in SN of Wistar adults rats, detected and semiquantified by multiplex RT-PCR. We observed the presence of the mRNA in SN by RT-PCR. Indirect immunofluorescence staining with anti-AT2 receptor primary antibody revealed AT2R localized in scattered immunoreactive cells AT2R might play an important role in neuroregeneration of damage cerebral areas. Additional research is required in order to further unravel the implications of the RAS in Parkinson disease.