DIFFERENT WAYS TO DETECT EXTRACELLULAR INFORMATION AND THEIR EFFECT ON THE RESPONSE DYNAMIC RANGE

JUAN PABLO DI BELLA; ALEJANDRO COLMAN-LERNER; ALEJANDRA C VENTURA

Rosario

Conferencia; SAIB - 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2014

SAIB

An important characteristic of every living cell is its ability to communicate with the surrounding environment. This exchange of information is called cellular signaling and is based on the capacity of the cell to give proper responses to environmental signals. Mathematical/computational modeling based on biological information can be used to improve our understanding of cellular signaling, thereby enhancing predictive accuracy. The first step in sensing extracellular information usually involves the reversible binding between an external ligand and a membrane receptor. We have shown in a recent publication that the dose-response curve of this sensing step (dose=ligand, response=ligand-receptor complex) is shifted to the high doses region when measured before reaching equilibrium, meaning that the utilization of extracellular information before ligand-receptor binding reaches equilibrium expands and shifts the input dynamic range (the dynamic range is the range of input concentrations that elicit distinguishable outputs). In this work we study how the properties of the ligand-receptor dose-response curve that allow PRESS are affected by adding complexity to the description of this sensing mechanism: consumption of ligand, receptor synthesis and internalization, and multiple receptor states.