Soy administered in hypercaloric diet modified the triglycerides of rat liver

RAZZETO GS, ESCUDERO NL, GIMÉNEZ MS

Mendoza

Congreso; Sociedad de Biologia de Cuyo; 2008

Sociedad de Biologia de Cuyo

Soy administered in hypercaloric diet modified the triglycerides of rat liver Razzeto GS, Escudero NL, Giménez MS Facultad de Química, Bioquímica y Farmacia. UNSL. San Luis. E-mail: grazzet@unsl.edu.ar Preliminary study in our laboratory show that administered hypercaloric diet to normal male Wistar rats produces changes on the lipidic metabolism The aim of this work is to evaluate the effect of vegetal protein (soy) respect to animal protein (casein) on the lipids in serum and liver when administered AIN-93G diet modified (hypercaloric). A lot of twenty male Wistar rats (21 days old) were separated in two groups: one control, fed with AIN 93G (CD), and a problem group was fed with the modified AIN-93G (containing 34,146% sucrose, 42% of calories from fat), during 9 weeks. Then they were separated in 4 lots: 2 lots of control group control casein (CC), control soy (CS) y 2 lots of problem group: casein hypercaloric (CH), soy hypercaloric (SH) and fed 45 days. At the end of the feeding period, animals were anesthetized and sacrificed. Serum and liver were isolated. frozen in liquid nitrogen. Total cholesterol (TC), cholesterol HDL, cholesterol LDL and triglycerides (TG) were determined in serum. In liver were measured total proteins, total lipids and total free and esterified cholesterol, TG and phospholipids (PL). The results showed that in serum TC decrease in CS (75.75 ± 4.86 mg/dL) in relation to CC (95.00 ± 5.66 mg/dL) (P< 0.05) and in liver TG decrease in SH (2949.73 ± 141.71 µg/g tissue) in relation to CH (4212.54 ± 690.31 µg/g tissue) (P< 0.05). The results indicate that protein vegetal could regulate the amount the TG when is administrated in a hypercaloric diet.