Crystal Structure and expression of a putative phage-like protein coded in the genome of a marine Antarctic bacterium (Poster)

LEONARDO PELLIZA; MARTIN ARAN; SEBASTIÁN KLINKE; JIMENA RINALDI; GABRIELA SYCZ; CLARA SMAL; GASTÓN PARIS; FERNANDO A. GOLDBAUM; DANIEL O. CICERO; JOSÉ LUIS LÓPEZ; BEATRIZ G. GUIMARÃES; ESTEBAN LANZAROTTI; ADRIÁN TURJANSKI; SUSANA VÁZQUEZ; WALTER MAC CORMACK

Ceske Budejovice

Conferencia; 6th International Conference on Polar and Alpine Microbiology; 2015

Centre for Polar Ecology, Faculty of Science, University of South Bohemia in Ceske Budejovice

Bizionia argentinensis JUB59T (B.a) was isolated from surface marine water in Potter Cove, (62 deg 14.9 min S; 58 deg 40.9 min W), Antarctica and described as a new species. JUB59 is a psychrotolerant marine bacteria; Gram-negative, nonmotile rods forming yellowish orange colonies on marine agar (Bercovich et al. 2008). The sequencing of JUB59T genome (GenBank AFXZ00000000) suggested interesting features such as UV resistance, hydrolytic exoenzymes, and nitrogen metabolism. Several putative gene sequences, of unknown biological function and with possible new folds were selected, cloned, expresed and its products were purified from the culture broth. One of the Ba gene products, named C24, showed structural homology with a T4 phage tail fiber protein. In this work, we investigate the structure and expresion of C24.