Cytotoxic effect of natural pseudoguainolides against lymphoma cells

SÜLSEN VALERIA; MARTINO RENZO; BEER FLORENCIA; ELSO ORLANDO; MARTINO VIRGINIA; ANESINI CLAUDIA

Talca

Congreso; 14 th International Congress of Ethnopharmacology; 2014

Cancer is one of the most important causes of death worldwide. According to the World Health Organization, lymphoma stand at the fifth place of cancer related death over the world. It is characterized by abnormal lymphocyte proliferation and appears as a solid tumor most commonly in the lymph nodes of the neck, chest, armpit or groin [1]. Chemotherapy is one of the best options in the treatment of cancer. However, currently used drugs have serious side effects. For this reason, it is necessary to search for new and more selective drugs with fewer adverse effects. Sesquiterpene lactones (STLs) are natural terpenoid compounds that have received considerable attention due to their antiparasitic, antiinflamatory and antitumoral activities. In this sense, the aim of the present study was to evaluate the effect of psilostachyin, psilostachyin C and peruvin, three STLs of the pseudoguaianolide type, on a lymphocityc leukaemia cell line (BW 5147). Psilostachyin C was the most active compound with a 50% effective concentration (EC50) of 4.89μg/mL when tested for its in vitro antiproliferative activity. This compound exerted cytostatic and cytotoxic activity in relation to apoptosis, but was non toxic when tested on normal murine lymphocytes (50% cytotoxic concentration (CC50) higher than 50μg/mL). These findings show the importance of STLs as potential candidates for lymphoma and leukemia treatment