EFFECTS OF CADMIUM EXPOSURE IN LUNG LIPIDS AND MORPHOLOGY

BOLDRINI, GABRIEL; ALVAREZ SILVINA M; PLATEO PIGNATARI MARIA G; PEREZ CHACA MV; BIAGGIO VERONICA S; MARTIN MOLINERO GLENDA D; GOMEZ NIDIA N; GIMENEZ MARIA SOFIA

ESTANCIA GRANDE, SAN LUIS

Congreso; REUNION ANUAL SOCIEDAD DE BIOLOGIA DE CUYO; 2014

SOCIEDAD DE BIOLOGIA DE CUYO

Cadmium (Cd) is a toxic metal and an important environmental contaminant. We studied its effects on the lipid profile and the histoarchitecture of rat lung under different diets. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water for 60 days. Lung homogenates were used for TBA assay, and the levels of lipid peroxidation products - mainly malondialdehyde (MDA) - were determined spectrophotometrically as TBARSs. Lipids were extracted; total cholesterol (TC), triglycerides (TG) and phospholipids (PL) were determined. Total RNA was isolated with Trizol and cDNA was obtained. Cytidylyltransferase (CT) was determined by PCR. S28 was used as the control.The lungs were fixed, sectioned, stained, and examined for evidence of lung injury. TBARS showed a significant increase (p