EFFECT OF TNFRP55 DEFICIENCY ON THE PG SYNTHESIS AT THE END OF PREGNANACY

DE LA VEGA M; RAGUSA JA; ANZULOVICH AC; CASAIS M

San luis

Congreso; XXXII Reunión Anual de la Sociedad de Biología de Cuyo; 2014

Sociedad de Biología de Cuyo

EFFECT OF TNFRP55 DEFICIENCY ON THE PG SYNTHESIS AT THE END OF PREGNANACY De la Vega M, Ragusa JA, Anzulovich AC and Casais M. Lab Biol Reprod (LABIR)-FQByF-UNSL, IMIBIO-SL-CONICET - San Luis. E-mail: magalidlv0@gmail.com.ar An adequate rhythm of progesterone is crucial for determining the physiological duration of the estrous cycle and for achieving a successful pregnancy. Tumor necrosis factor is a pleiotropic cytokine and TNF receptor p55 (TNFRp55) mediates most of the TNF effects. We previously showed that Progesterone (Pg) levels and synthetic enzyme (3β-HSD) exhibit a circadian rhythm in the ovary in diestrus. In this work we investigate the impact of TNFRp55 lack in the Pg metabolism in the end of pregnancy in C57BL/6 wild type (WT) and TNFRp55-/- (KO) mice. Animals were maintained on a 12-h light: 12-h dark cycle, at 24±2°C, with irradiated food and water available ad libitum. Five days before the experiment mice were kept under constant darkness conditions. Ovaries were isolated every 6 h during a 24h period. Pg and 3β-HSD protein levels were determined by RIA and Western Blot respectively. The same as that observed in diestrus, Pg levels in late pregnancy are circadian in the ovary of WT mice (p