EFFECTS OF CADMIUM EXPOSURE IN CEREBELLUM ? EFFECT OF DIETS AND AGE

ALVAREZ SILVINA M; PLATEO PIGNATARI MARIA G; PIGUILLEM SILVANA; VALDEZ SUSANA R; BIAGGIO VERONICA S; GIMENEZ MARIA SOFIA

ESTANCIA GRANDE, SAN LUIS

Congreso; REUNION ANUAL SOCIEDAD DE BIOLOGIA DE CUYO; 2014

SOCIEDAD DE BIOLOGIA DE CUYO

Cadmium (Cd) is a toxic metal and an important environmental contaminant. We studied its effects on the oxidative stress markers and the histoarchitecture of rat cerebelum under different diets. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water for 60 days. On the other hand, we also measured some oxidative markers in rat fetus´s cerebellum whose mother received Cd during the pregnancy. Cerebellums were isolated and total RNA was obtained with Trizol. Nrf 2, NOX and SOD were determined by RT-PCR. S28 was used as the control. Histological studies were performed in fixed Hematoxilin-Eosin-stained tissues. In adult rats, nrf2 and SOD decreased in Cd groups (p0.05) and that SOD increased only in Soy-Cd (p>0.05). These results suggest that in the fetuses the response to oxidative stress induced by Cd is better than the one found in adult rats, where the antioxidant system is depressed. Further studies are necessary in order to learn the effect of Cd on the fetuses? cerebellum oxidative stress metabolism and architecture.