Influence of nutritional conditions on antibacterial activity of Bacillus sp SL-6

COZZOLINO M; DISTEL J; DIP E; SILVA P

Córdoba

Congreso; III Reunión Internacional de Ciencias Farmacéuticas; 2014

In the last years, antibiotic development continues stagnate, so that the isolation of antibiotic-producing microorganisms from different environments became relevant in pharmaceutical, agronomic and food industries. Bacillus species are considered as ?cell factories? producing a large number of bioactive compounds against bacteria, fungi, protozoa and viruses. This report studies the effect of different culture media and several carbon sources of a chemically defined broth in the estimation of antibacterial activity of Bacillus sp SL-6. Bacillus sp. SL-6 was grown in Synthetic Mineral Broth (SMB) with orbital shaking at 200 rpm for 24 h at 30ºC. Glucose was replaced by glycerol, lactose, sucrose, maltose and starch in SMB at a final concentration of 10g/l. Samples were centrifuged and filtered to obtained cell-free supernatants (CFS). Screening of antibacterial activity of SCFS on Mueller-Hinton, Brain Heart Infusion, Tryptone Soy Agar, Plate Count Agar and Glucose Peptone Agar (GPA) was tested against Staphylococcus aureus ATCC 29213 and Yersinia enterocolitica W1024 by agar well diffusion method. The detection of anti-Yersinia activity was clearly manifested in all solid media used. In contrast, the anti-Sthaphylococcus activity showed hazy inhibition, with exception of observed activity on GPA. CFS of cultures in SMB supplemented with glucose and glycerol gave maximum anti-Staphylococcus activity, while that those added of maltose and lactose showed activity only against Y. enterocolitica. No significant variation (p