INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Identification by LC/ESI/MS/MS and LC/DAD of flavonol and isoflavonoid glycosides modulated by solar UV-B radiation and herbivory in soybean leaves at field: effects on Anticarsia gemmatalis larvae.
Autor/es:
DILLON, F; CHLUDIL, H; ZAVALA, J. A.
Lugar:
Bogota
Reunión:
Congreso; III Congress of the Latin American Association of Chemical Ecology (ALAEQ); 2014
Institución organizadora:
Latin American Association of Chemical Ecology (ALAEQ)
Resumen:
Solar ultraviolet B (UV-B) radiation has been
largely reported to enhance plant defenses against insects, but it is unclear
what traits define insect resistance at field in crop systems such as soybean.
Here we study the effects of solar ultraviolet B (UV-B) radiation on: a) the
modulation of phenolic compounds and trypsin inhibitors (TPI) in soybean leaves
b) survival and mass gain of Anticarsia
gemmatalis larvae in field conditions.
Cultivar (cv.) Williams and cv. Charata were
planted at field under two treatments generated by thick plastics: ambient UV-B
and attenuated UV-B. Neonates of A.
gemmatalis larvae were placed in transparent tull bags on the last fully
expanded soybean leaves and the number of surviving larvae was recorded daily
and at day six weighted. Flavonoids glycosides from soybean leaves were
identified by HPLC-DAD and HPLC-ESI(+)-Q-TOF
Exclusion of UV-B radiation increased mass gain
of larvae fed on cv. Charata, however this changes were not that important in
cv. Williams. TPI activity, genistin, malonyl genistin and a benzoic acid
derivate were induced by A. gemmatalis
damage in both cultivars, but only in cv. Williams this induction was more
intense in plants that grown under solar UV-B radiation. TPI activity and
genistein derivatives didn´t explain the differences found in survivorship and
mass gain of larvae that fed on cv. Charata in the two UV-B treatments. However,
after identification and quantification of flavonols present in soybean leaves
(tri and diglycosides of quercetin, isorhamnetin and kaempferol) we found an
association between modulation of plant resistance to herbivory and two
quercetin triglycosides present only in cv.Charata.
We found that modulation of two quercetin
triglycosides were more important in defining larvae performance than the
modulation of other typical defenses such as TPI activity and genistin. We also
identified a benzoic derivate which was induced by herbivory independently of
UV-B radiation.