Toxicological Analysis of Rosemary Bioactives with Potential Use in Clinical Applications against Bacteria-Resistant in Rabbits

OJEDA-SANA AM ; CÁCERES GUIDO PA; ASPREA M; VAN BAREN C; BALBARREY Z; ADRIANA MACCHI; FILIPPO D.; MORENO S.

La Habana

Congreso; XX Congreso Latinoamericano de Farmacología y Terapéutica, V Congreso Iberoamericano de Farmacología, V Congreso Internacional y XI Congreso Nacional de la Sociedad Cubana de Farmacología; 2013

Sociedad Cubana de Farmacología

Introduction: this work documented plant drug exposure of rosemary bioactives in the skin of rabbits. Previously, we demonstrated the pharmacological potential of several rosemary compounds in vitro and in two skin infection models in mice against human pathogenic bacteria1-4. However, remains to investigate if they have no toxicological effects on the skin. The aim of this study was to evaluate induction of irritation and/or dermal corrosion, and inflammatory reactions by contact with the skin of the rosemary bioactive in rabbit. Materials and methods: the protocol included a three topical dose at 0hs, 24hs and at 48hs. Toxicity was assessed by determination of erythema at 1h, 24hs, 48hs and 72hs of treatment on four skin areas of 4 cm2 on each animal by photographic analysis. The edema was investigated by ultrasound analysis (echography) of the dermis and quantified by image analysis. The animals were euthanized after 72hs and histopathological analyzes of the skin treated with the topical cream containing the bioactive or vehicles were performed in biopsy specimens stained with hematoxylin eosin. Also, pharmacokinetic parameters of plant compounds were studied in the serum of the animals by gas chromatography after 30min, 1h or 24h of treatment. Results: the skin treated with the plant compounds did not show significant signs of swelling redness, blisters, peeling or ulcerations after 4 days of treatment and three consecutive doses. In addition, skin areas showed the same thickness in the groups treated with the active plant and treated with the vehicle (1.50 + 0.22 mm vs. 1.81 + 0.28 mm) after ultrasound examination.   Conclusions: this work has a direct impact on areas of health, since we developed innovative formulations based in herbal alternatives, not only effective but also safe, for the treatment of bacterial resistant infections.1Free Rad Res 2006,40:223; 2BLACPMA 2009,8(3):219; 2Pediatric Res 2012,72(1):109; 3Food Control 2013,31:189.