EFFECT OF CADMIUM EXPOSURE ON LIPIDIC ENZYMES ACTIVITY IN RAT CEREBELLUM ? COMPARISON BETWEEN DIFFERENT DIETS

ALVAREZ SILVINA MONICA; PLATEO PIGNATARI MARIA GABRIELA; GIMENEZ MARIA SOFIA

Mendoza

Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2013

Sociedad de Biologia de Cuyo

EFFECT OF CADMIUM EXPOSURE ON LIPIDIC ENZYMES ACTIVITY IN RAT CEREBELLUM ? COMPARISON BETWEEN DIFFERENT DIETS Álvarez SM, Plateo Pignatari MG and Giménez MS. Lab de Nutricion y Medio Ambiente, IMIBIO-CONICET. San Luis. Argentina. salvarez@unsl.edu.ar Cadmium (Cd) is a toxic element and an important environmental contaminant. We studied its effect on the lipid profile and lipidic enzymes of rat cerebellum under different diets. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water for 60 days. Lipids were extracted and separated by TLC. Total cholesterol (TC), triglycerides (TG) and phospholipids (PL) were determined. Total RNA was isolated with Trizol and cDNA was obtained. Hydroxymethylglutaryl CoA Reductase (HMGCoAR), Acetyl CoA Carboxilase (ACC), Fatty Acid Synthase (FAS), Glycerol 3 phosphate acyltransferase (GPAT) and Diacylglycerol Acyltransferase (DGAT) were determined by PCR. S28 was the control. TC showed an increase in the Cd groups (p