MALE INFERTILITY IN OBESE C57BL/6J MICE: CORRELATION BETWEEN REDOX/INFLAMMATION STATUS AND TESTICULAR MORPHOLOGY

ALTAMIRANO, L ; DELLA VEDOVA, MC; SANDRA E. GOMEZ-MEJIBA; DARIO C. RAMIREZ; FORNES, MW

SANTIAGO

Congreso; XXIV REUNION ANUAL DE LA SOCIEDAD CHILENA DE REPRODUCCION Y DESARROLLO; 2013

SOCIEDAD CHILENA DE REPRODUCCION Y DESARROLLO

INFERTILITY IN A MODEL OF DIET-INDUCED OBESITY IN MALE B6 MICE Altamirano L, Simón L, Sáez E, DellaVedova MC, Gomez-Mejiba SE, Ramírez DC y Fornés MW. mfornes@fcm.uncu.edu.ar LIAM (IHEM), FCM ? UNCuyo, CCT CONICET - Mendoza and Laboratorio de Medicina Experimental & Terapéuticas, IMIBIO-SL-CONICET-UNSL, San Luis. Argentina. Obesity and overweight are known causes of infertility in men, but the mechanism involved remains unknown. The aim of this study was to evaluate the redox profile/ inflammatory/metabolic systemic and testicular morphological abnormalities that could explain the sub-fertility. Mice model included 2 groups: LFD (low fat diet) and HFD (high-fat diet). At 18 weeks HFD group showed an increase in proinflammatory cytokines (TNF-a and IL-6) and oxidative stress markers (carbonyl and TBARS) levels. Basal fasting blood glucose and intraperitoneal glucose tolerance tests showed insulin resistance in HFD group. Epididymal fat weight was greater in HFD, and testicles showed foam cells in the interstitium. The spermato/spermiogenesis showed patchy epithelial disorganization, and abnormal spermatocytes with abnormal acrosome complex-acroplaxoma. These data suggest that the abnormalities found in spermatogenesis may be caused by changes in redox/inflammatory/metabolic status in testis, reflecting the systemic situation associated to inflammation of the adipose tissue.