Sequence evolution of the intrinsically disordered and globular domains of the adenovirus E1A oncoprotein.

GLAVINA J; SÁNCHEZ IE

Puerto Varas

Congreso; 2013 Latin American Protein Society Meeting; 2013

Latin American Protein Society

The small DNA tumor virus adenovirus induces cell transformation in several animal models by the action of the Early 1A and 1B oncoproteins. The HAdV5 E1A gene encodes 2 major proteins that only differ in an internal sequence of 46 residues. The larger E1A consist of 5 regions: The less conserved Nterminal region and four conserved regions designated CR1 to CR4. CR3 is absent in the smaller E1A. The CR3 is predicted to be globular and mediates zinc binding. The N-terminal, CR1, CR2 and CR4 are intrinsically disordered and harbor several linear motifs that mediate interaction with several cellular targets. We analyzed disorder and sequence conservation in over 100 E1A sequences.