Nuevos Biomateriales de Silicio y Colágeno.

FOGLIA M LUCIA; CAMPOROTONDI DANIELA EDHIT; ALVAREZ GISELA SOLANGE; DÍAZ LUIS EDUARDO; DESIMONE MARTIN FEDERICO

Jornada; XIII Encuentro de Superficies y Materiales Nanoestructurados; 2013

Silica-collagen scaffolds were obtained by covalent binding of an aminosilane to glutaraldehyde fixed collagen hydrogels, rendering a three dimensional network of silicon coated collagen fibrils. When compared to non silicified collagen, silica containing matrices exhibited a 60 fold increment in the rheological properties. Moreover, acellular degradation by collagenase type I indicated that enzymatic digestion occurred at a slower rate for silica modified hydrogels, hence enabling a controlled degradation of the obtained material. In addition, fibroblastic cells seeded on silicified collagen matrices were able to adhere, proliferate and migrate within the scaffold for over 3 weeks as shown by MTT tests and hematoxylin-eosin staining. These results suggest that the herein described method could be useful in the design of materials for tissue engineering purposes.