INVESTIGADORES
BABOT Jaime Daniel
congresos y reuniones científicas
Título:
Lactic acid bacteria isolation from chicken intestinal content and selection of strains with capacity to capture toxic dietary lectins.
Autor/es:
JAIME DANIEL BABOT; ELOY ARGAÑARAZ MARTÍNEZ; MARÍA CRISTINA APELLA; ADRIANA PEREZ CHAIA
Reunión:
Simposio; 10th Symposium on Lactic Acid Bacteria; 2011
Resumen:
The diet components of laying
hens and broilers contain secondary products of plant metabolism, lectins among
them, which act as a defence mechanism in vegetables. The presence of dietary
lectins may impair intestinal epithelia development and enzyme digestive
activity in baby chicks with the consequent growth depression. Lactic acid
bacteria (LAB) may decrease the cytotoxic effects of lectins on intestine
epithelial cells capturing these glycoproteins by interaction with bacterial
surface carbohydrates. Hence, the aim of this work was to isolate, identify and
select LAB strains with capacity to survive the gastrointestinal transit and
capture toxic dietary lectins. In addition, 9 Propionibacterium strains previously isolated from the same niche
were assayed. LAB strains were isolated from chicken gastrointestinal tract using HHD, LBS and KF agar
media and identified by 16S rDNA gene sequencing. In order to analyze their
ability to grow at 42 °C, growth kinetics of all strains were studied in LAPTg
+ 1% lactose, MRS or LAPTg broth depending on the isolation media. Then, the
strains were subjected to an in vitro
simulated gastrointestinal digestion, performed at 42 °C. Finally, their ability
to capture lectins (DBA, ConA, PHA-P, PNA, UEA-I and WGA) was studied
fluorometrically. Eighty one LAB strains were isolated from gastrointestinal content of
laying hens; 58 of these strains grew at 42 °C. Viable counts of 14 LAB
and 4 propionibacteria strains decreased less than a log unit after the simulated
gastrointestinal digestion. Thirteen strains bound at least one lectin, being WGA
and ConA caught by most of the strains. These strains may be used as probiotic
in order to protect baby chicks intestinal epithelium in diets with high
content of lectins.