“RAT OVARY EXPOSED TO CADMIUM: EFFECT OF SOYBEANS AS PROTEIN SOURCE”

CALDERONI AM, ALVAREZ SM, PLATEO PIGNATARI MG, PEREZ DIAZ MG, GIMENEZ MS.

San Luis

Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biologia de Cuyo

Cadmium (Cd) is a toxic element considered as an important environmental contaminant, and a major constituent of tobacco smoke. The aim was to study the effect of chronic exposure to cadmium on the lipidic profile of rat ovaries and the incidence of different diets: one casein based and one soybean based. 4 lots of adult female Wistar rats were formed: 2 lots received casein and 2 lots soybeans as protein source in the diet. Within each protein group, 1 lot received water without Cd (control) and the other received 15 ppm of Cd (as Cl2Cd) in the drinking water for 60 days. Lipid extraction from ovaries was made with isopropanol: hexane 3:2. Lipids were separated by TLC using hexane : ethylic ether : acetic acid 80:19:1. We measured total cholesterol (TC), triglycerides (TG) and phospholipid contents (PL). Proteins were measured by Lowry. The results are media ± SEM. TC (g/mg protein): Casein Control: 5.9790.44; Casein Cd: 18.361.62; Soy Control: 21.18±3.17 and Soy Cd: 14.79±0.07 (p