"CADMIUM EXPOSURE ON RAT CEREBELLUM. EFFECT OF DIFFERENT DIETARY PROTEINS"

ALVAREZ SM, CALDERONI AM, PLATEO PIGNATARI MG, PEREZ DIAZ MG, GIMENEZ MS

San Luis

Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biologia de Cuyo

Cadmium (Cd) is a toxic element and an important environmental contaminant. We studied its effect on the lipid profile and antioxidant enzymes of rat cerebellum under different diets. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water for 60 days. Lipid extraction was made with isopropanol: hexane 3:2 and were separated by TLC. We measured total cholesterol (TC), triglycerides (TG) and phospholipids (PL). Proteins were measured by Lowry. Total RNA was isolated with Trizol and cDNA was obtained. Catalase (CAT), Superoxide Dismutase (SOD) and NF-E2 related nuclear factor (nrf-2) were determined by PCR. Beta actin was the control. TC showed an increase in the Cd groups (p