Effect of Cadmium on Glutathione Metabolism and PPAR Expression in Small Intestine

LARREGLE E, CEVALLOS C, FERNANDEZ MR, VARAS S, GIMENEZ

Congreso; Sociedad de Biologia de Cuyo; 2012

Effect of Cadmium on Glutathione Metabolism and PPAR Expression in Small Intestine Larregle E, Cevallos C, Fernandez MR, Varas S, Gimenez MS. Área de Química Biológica, Fac. Qca, Bioqca y Fcia. Proyecto 8104. UNSL. E-mail: elarre@unsl.edu.ar Cadmium (Cd) is an important environmental contaminant that induces oxidative damage in cells. Previous results obtained in our laboratory indicate that Cd present in small intestine (SI) produced lipoperoxidation and altered expression of inflammation factors. Cd, TBAR’s, MT content increased, CAT and GPx activities and SOD, GPx and NOX expression did not change, TNFα, PPARγ and Nrf2 expression increased, Glutathione Reductase activity (GR) decreased in SI of rats treated with Cd via oral. The objective of this work was determinate if Cd affects the glutathione metabolism and the expression of other PPARs. Wistar male rats (180g) were treated with Cd (15 ppm) in tap water during 2 months. GSH content and the activities of enzymes related to NADPH production was measured. The expression of PPARα and β, and β-actin (internal control) mRNA levels, were determine by RT-PCR. Tissue total RNA was isolated using TRIzol. The PCR products were analyzed on 2% agarose gels containing Gel Red. Band intensities of RT–PCR products were quantified using Image J software. GSH and G6PDH showed a decrease, ICDH did not change, PPARα and β expression increased in SI respect to the control (P