CADMIUM EXPOSITION ON REDOX BALANCE IN AORTA. EFFECT OF SOY BEAN AS PROTEIN SOURCE IN THE DIET

MATIAS FEDERICO PEREZ DIAZ; FERRAMOLA MARIANA; LILIANA BEATRIZ OLIVEROS; GIMENEZ MARIAS SOFIA

San Juan

Congreso; Segunda Reunión Conjunta de Sociedades de Biología de la República Argentina; 2011

Sociedad Argentina de Biología, Sociedad de Biología de Cuyo y Sociedad de Biología de Cordoba

Cadmium (Cd) is one of the most toxic pollutants. Its toxicity has been associated to cardiovascular alterations. Here, we study the oxidative effects of Cd in aorta and the antioxidant activity of soybeans, as protein source in the diet, as a preventive agent of redox alterations caused by Cd. 6 lots of adult male Wistar rats were conformed to 6 rats each. 3 lots received casein and 3 lots soybeans as protein dietary source, respectively. Within each protein group, 3 lots were conformed: one received water without Cd (control group) and the other two received 15 and 100 ppm of Cd (as Cl2Cd) in the drinking water, respectively, for 60 days. In toraxic aorta the content of thiobarbituric acid reactive substance (TBARS), as indicator of lipoperoxidation, and the activities and protein levels (Western blot) of enzymes involved in the redox status were determined. Aorta of rats exposed to 100 ppm Cd and dietary casein showed an increase of NAD(P)H oxidasa (NOX-2) protein, activity and protein expression of catalase (p