IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
INFLUENCE OF HYPOTHYROIDISM ON LIPID METABOLISM IN THE HEART OF VIRGIN RAT
Autor/es:
CARMONA Y; MARRA C; OLIVEROS L; GIMENEZ MS
Lugar:
SAN JUAN
Reunión:
Congreso; Second Joint Meeting of the Biology Societies from Argentina. - ARGENTINA.; 2011
Institución organizadora:
Sociedades de Biología de la Republica Argentina
Resumen:
Thyroid
state influences cell and plasma lipid composition. The
aim of this work was to determine the effects of hypothyroidism on lipid
metabolism in the heart of female virgin Wistar
rats, weighing 150-180g. Hypothyroid state was induced by administration
of 6-n-propyl 2-thiouracil (100 mg/L) in
the drinking water, for 30 days. Age-matched euthyroid rats were used as
controls. Serum lipids were measured by Wiener kits and heart lipids by
spectrophotometric assays after lipid extraction (Folch method) and separation of the different
lipid fractions (thin-layer chromatography). mRNA expressions of peroxisome proliferator-activated receptor-α (PPARα) and sterol regulatory element binding protein 1c (SREBP-1c) were determined by RT-PCR,
using β-actin as internal control. Heart fatty acid composition was determined by gas-liquid chromatography. Hypothyroid state
induced an increase of serum cholesterol and LDLc, a decrease of serum and
heart triglycerides (TG) (p<0.01), and an increase of the heart fatty acid
unsaturation index (UI). Heart mRNA levels of PPARα was decreased (p<0.01), without
change in SREBP-1c, in relation to control. Hypothyroid state alters serum and
heart lipids content, and decreases the expression of PPARα, which is involved
in the regulation of heart energetic balance. In addition, the high UI suggests
a low protection against fatty acid oxidation in heart.