INTEC   05402
INSTITUTO DE DESARROLLO TECNOLOGICO PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Study of different supports and activation protocols for Beta-galactosidase from Kluyveromyces lactis immobilization
Autor/es:
BEZERRA, CAMILLA; DE SOUZA, MARIA CRISTIANE; DE FREITAS, WENDEBERTO; MAMMARELLA, ENRIQUE; GONÇALVES, LUCIANA; ADRIANO, WELLINGTON
Lugar:
Buzios
Reunión:
Congreso; XIX Congresso Brasileiro de Engenharia Quimica; 2012
Institución organizadora:
Associação Brasileira de Engenharia Química e Universidades UFRJ, UERJ, UFRRJ e UFF
Resumen:
Chitosan,
cashew bagasse and coconut husks are raw materials found in northeastern Brazil
as industrial waste presenting low cost and used as alternative supports for
enzymes immobilization. β-galactosidase from Kluyveromyces lactis, EC
(3.2.1.23) has received a particular interest because it can be used to produce
a molecular mixture of glucose and galactose from lactose. This work aims
studying activation methods supports for β-galactosidase immobilization.
Glutaraldehyde activation was carried out by two ways. Support was added to
glutaraldehyde 5% v/v for 30 min, pH 7.0 (GLU1) or it was added to KOH 0.5 mol.L-1 for 30 min followed by dialdehyde addiction until 0.8% v/v for 30 min at
50°C (GLU2). The same was done with epichlorohydrin (EPI) or glycidol (GLY). However,
it was performed an activation by using glycidol (DFGLY) or epichlorohydrin (DFEPI) with previous support
treatment using dimethylformamide and 0.1 mol.L-1 KOH during 18h. Enzyme activity was measured using lactose 5% m/v as
substrate and glucose concentration determined by GOD-PAP method. Offered
enzyme loading in potassium phosphate buffer pH 7.0 with MgCl2 and MnCl2 was added to the activated support at 25°C for 3h. Some of
derivatives showed 100% of immobilization yield (IY). Best derivative was chitosan GLU2 with 100% and 19.2% of recovered activity. According to this work, use
of chitosan for β-galactosidase immobilization becomes an attractive
alternative for lactose hydrolysis at dairy industry.