Comparison of the effectiveness between STR and SNP panels for forensic DNA analysis in bovine breeds

GIOVAMBATTISTA G

Cairns

Congreso; 33rd Conference of the International Society of Animal Genetics; 2012

Society for Animal Genetics

STRs have been successfully used in animal genetic identifcation in forensic investigations, however in the last years, SNPs have gained traction. An effcient SNP identifcation system requires a marker set with enough power to identify individuals. In this study, information obtained from SNPs and STRs in Taurine and Cebuine breeds was compared. Samples (n = 378) from 15 breeds and 7 forensic caseworks were genotyped using18 STRs and 32 SNPs. The effects of quality, amount, and tissue sources (blood, hair, bone, beef) of DNA on genotype performance were evaluated. The SNP results showed a performance of 81.1% of call rate and a percent concordance between both replicates of 98.7%. These results were mainly explained by 2 factors: that assay design, and DNA samples quality rather than DNA quantity were important. Cumulative SNPs exclusion power values for sample matching, using double exclusion criteria, within each breed showed that in Taurine breeds information from the analyzed SNPs are equivalent to the 12 STRs ISAG recommended set (~10?11). In Cebuine breeds, this set exhibited only a match probability between ~10?7 and ~10?9. The test also showed a range of 61 to 99% of correct assignment within each breed. These results could provide a valuable population data that support the consensus SNP panel for bovine genetic identifcation.