IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PRENATAL TREATMENT WITH CAPTOPRIL INDUCES EMPHYSEMA IN RAT LUNG DEVELOPMENT
Autor/es:
CAPELARI, DN; SANCHEZ SI; FUENTES LB; ORTEGA HH; CIUFFO GM
Lugar:
San Juan
Reunión:
Congreso; Reunion Anual Soc. de Biologia de Cuyo; 2011
Institución organizadora:
Soc. de Biologia de Cuyo
Resumen:
Angiotensin-converting enzyme inhibitors (ACEI) have been widely
used in hypertension treatment. However, the use of ACEI during
pregnancy is contraindicated for risks of fetopathy. We investigated
the effect of captopril prenatal treatment in lung development. Wistar
rats were treated with captopril (2,85 mg/kg/day) delivered subcutaneously
with osmotic mini-pumps during the last week of pregnancy.
Pups lungs at four different postnatal ages (P0, P8, P15 and
P30) were evaluated by morphological, histomorphometric and
immunohistochemistry staining analysis. Captopril treatment significantly
decreased body and lung weights at P0 and P8. Histological
study evidenced a substantial destruction of alveolar walls
in treated rats, resulting in enlargement of distal airway spaces at
P8, P15 and P30, demonstrated by significant increase in the
interalveolar wall distance, quantified by mean linear intercept
(MLI). Cellular proliferation was evaluated using PCNA (proliferating
cell nuclear antigen). The percentage of PCNA-positively
stained alveolar epithelial cells in the treated group was significantly
higher at P15 and P30, respect to the control group. Cell proliferation
decreased with age in control animals. However, in captopriltreated
lungs the relative number of PCNA immunopositive nuclei
remains high during the studied ages. These findings support the
hypothesis of a functional renin-angiotensin-system required for
normal lung development.