Effect of trophoblast cell exosomes on human hofbauer cells phenotype and function

BRENDA LARA; DAIANA VOTA; GUILLERMINA CALO; GUSTAVO IZBIZKY; ESTEBAN GRASSO; HAUK, VANESA; FATIMA MERECH; J. IGNACIO ABASOLO; CLAUDIA PEREZ LEIROS; DANIEL PAPARINI; SCHAFIR, ANA; MARIA SOLEDAD GORI; M. AGUSTINA ARSLANIAN; RAMHORST ROSSANA

Mar del Plata

Congreso; LXVII Reunión anual de la Sociedad Argentina De Investigación Clínica (SAIC).; 2022

SAIC SAI SAFIS FAIC

Hofbauer cells (HBC) are the only foetal immune cell population within the stroma of healthy placenta. However, the functional properties of these cells are poorly described. HBCs are transcriptionally similar to yolk sac macrophages. Trophoblast cells release exosomes (Ex) that regulate target cell function during pregnancy. Here we studied the profile of HBC modulated by trophoblast exosomes, physiological or pathological stimuli. Methodology: Trophoblast cell line (Swan 71-Tb) Ex were obtained by differential centrifugation and characterized. HBC were isolated from human term placentas (N= 25) by enzymatic digestion. They were cultured with 75-100 ng/ml Tb-Ex for 18 h to assess phenotypic profile, glucose uptake and lipid droplets formation by flow cytometry. 100 nM vasoactive intestinal peptide (VIP) or 100 ng/ml E. coli lipopolysaccharide (LPS) were used as anti or proinflammatory stimuli. HBC supernatant was used to study endothelial cell (EC) migration by wound healing assay.Results: Tb-Ex increased antiinflammatory marker CD39 (*P