Effects of hypothyroidism on oxidative stress in heart of pregnant rats.

CARMONA Y, OLIVEROS L, GIMENEZ MS.

Mendoza

Congreso; Reunión Científica Conjunta de la Sociedad de Biología de Cuyo; 2010

Sociedad de Biología de Cuyo,

Hypothyroidism is associated to alterations in physiological functioning of heart muscle. Various studies on the effects of thyroid status on heart oxidative stress have produced conflicting results. In this work we studied the effect of hypothyroidism on oxidative stress in ventricles of heart from pregnant rats. The hypothyroid state was induced by 6-n-propyl-2-thiouracil (100mg/g body wt) in drinking water given to female Wistar rats (150-180 g body wt) starting 8 days before mating until day 21 of pregnancy (HP group) or for 30 days in virgin rats (HV group). The mRNA expressions (by RT-PCR, using β-actin as internal control) and activities of NADPHoxidase (Nox2), superoxide dismutase (SOD), catalase (CAT) and gluthathione peroxidase (GPx) were measured in the heart. Also, TBARs (as MDA) and carbonyl groups content were determined. In HP rats, the hypothyroidism did not have an effect on all these parameters, while in HV rats the hypothyroidism increased carbonyl content and GPx activity (p