CONCENTRATION OF FATTY ACIDS ALTERATIONS IN MAMMARY GLAND OF VITAMIN A DEFICIENT RATS

VASQUEZ ME, MARRA C1, GIMENEZ MS.

mendoza

Congreso; XXVIII Reunión científica anual de la sociedad de biología de cuyo; 2010

SOBIO

CONCENTRATION OF FATTY ACIDS ALTERATIONS INMAMMARY GLAND OF VITAMIN A DEFICIENT RATSVasquez ME, Marra C1, Gimenez MS.Dept. Bca y Cs Biológicas, UNSL, IMIBIO-SL CONICET, 5700San Luis, 1INIBIOLP, CONICET, La Plata, Bs As. E-mail:eridnere@hotmail.comOur objective was determined if the vitamin A deficiency altersthe quality and concentration of fatty acid in mammary gland.Wistar female virgin rats were separated at weaning into 3 groups:1) fed with vitamin A sufficient diet (+A, control) for 90 days, 2)vitamin A deficient diet (-A) for 90 days, and 3) vitamin A deficientdiet for 75 days follows by realimentation with vitamin Asufficient for 15 days (Ar). In other experimental model the 3groups were: 1) +A diet for 180 days, control, 2) –A diet for 180days and 3) –A for 175 days and realimentation with +A for 15days. The lipids were extracted by Folch method. After dried undernitrogen the extracts were saponified and processed to preparethe fatty-acyl methyl esthers (FAMEs) using a capillary column.Before injection into the c-GLC system, the FAMEs werepurified by HPTLC chromatography. The patterns of the FAMEswere identified by comparison of their relative retention timeswith authentic standards running in parallel. Relative mass distributionof each analysis was calculated electronically by quantificationof the peak areas. In both experimental models the vitaminA deficiency caused a decrease of 18:0, 18:2n-6, 20:5n-3 and 22:5n-3, and an increase of 18:1n-9, 22:4n-6, 22:5n-6 fatty acid concentrations.The realimentation with vitamin A reverted thosevalues to +A control values. The data confirm that the fatty acidalterations were produced by the vitamin A deficiency.