Valorization of corn cob for the obtention and purification of endoglucanase produced by SSF

MARÍA BELÉN ALLASIA; CRISTOBAL AGUILAR; BOGGIONE, MARÍA JULIA; BEATRIZ FARRUGGIA

PROCESS BIOCHEMISTRY - (Print)

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2020 p. 106 - 112

1359-5113

ABSTRACTAgro-food by-products contain valuable nutrients which are wasted. In this work, solid-state fermentation (SSF)was carried out using corn cob as substrate for endoglucanase production. The radial growth of three fungalstrains -Trichoderma harzianum T104, Aspergillus niger GH1 and Aspergillus niger NRRL3- was analyzed in order toselect the most appropriate. Radial growth data were analyzed with a mixed linear model for longitudinal dataand no statistically significant differences were found between both A. niger strains.Endoglucanase was separated from the extract of A. niger GH1 by fast protein liquid chromatography(FPLC).The highest endoglucanase activity was detected in fraction number three collected from FPLC corre-sponding to 72 h of SSF. Seven bands in a range from 24 to 50 kDa, which correspond to endoglucanase fromfungal extract, were detected by zymogram analysis. According to protein quantification performed by theImageJ software, 85% of the proteins present in the samples collected by FPLC corresponded to endoglucanaseproteins. The purified endoglucanase retained about 100% of its catalytic activity at 30 °C and 50 °C and wasstable in a pH range between 4.00-6.00. These properties make this isolated enzyme suitable for industrialapplications such as the saccharification process for bioethanol production.