SYNTHESIS OF POLYMERIC MATRICES FOR ADSORPTION AND PURIFICATION OF ENDOGLUCANASE

MARÍA BELÉN ALLASIA; PAULA ZILLI; MARÍA JULIA BOGGIONE; BEATRIZ FARRUGGIA

JOURNAL OF POLYMERS AND THE ENVIRONMENT

SPRINGER/PLENUM PUBLISHERS

Lugar: New York; Año: 2018 vol. 26 p. 4321 - 4330

1566-2543

Chitosan (CHS), chitosan?Eudragit® EPO (CHS?EPO) and chitosan beads partially cross-linked with glutaraldehyde (CHS?GLUT) were obtained in order to improve the adsorption selectivity of endoglucanase from a fungal culture obtained underSSF by Aspergillus niger using soybean harvest residues as support. The matrices synthetized were characterized in termsof physical and chemical changes. Fourier-transformed infrared spectroscopy with attenuated total reflectance device (FTIRATR)was employed to verify the chemical changes on the CHS matrix after the synthesis of CHS?GLUT and CHS?EPO.Scanning electron microscopy (SEM) was performed to compare the surface morphology of the polymeric beads. Twovariables, purification factor and yield percentage of the adsorption process, were analyzed using a bifactorial ANOVAconsidering the matrix?time first order interaction. SEM results exhibited greater surface roughness in the CHS?GLUTand CHS?EPO matrices which may enhance endoglucanase adsorption. FTIR-ATR results confirmed an effective chemicalmodification of the CHS matrix after crosslinking with GLUT and corroborated the efficiency of the synthesis of the CHS?EPO matrix by the presence of chemical groups of the EPO polymer. An endoglucanase purification factor close to 9 wasachieved with the CHS?GLUT matrix and a yield percentage of 60% was obtained with the CHS?EPO matrix. BifactorialANOVA results showed the matrix?time interaction to be significant for both variables. The CHS?GLUT matrix with lowcrosslinking times and the novel CHS?EPO matrix could be included in the bioseparation stage of endoglucanase using asimple and a low-cost method such as batch adsorption.