3D electron tomographic and biochemical analysis of ER, Golgi and trans Golgi network membrane systems in stimulated Venus flytrap (Dionaea muscipula) glandular cells

STAEHELIN, L. ANDREW; DONOHOE, BRYON S.; HERDER, RACHEL; MARTINEZ, DANA E.; MOGELSVANG, SOREN; GERGELY, ZACHARY R.

Journal of Biological Research-Thessaloniki

BMC Springer Nature

Lugar: Londres; Año: 2018 vol. 25 p. 1 - 16

The insect-trapping leaves of Dionaea muscipula provide a model for studying the secretory pathway of an inducible plant secretory system. The leaf glands were induced with bovine serum albumin to secrete proteases that were characterized via zymogram activity gels over a 6-day period. The accompanying morphological changes of the endoplasmic reticulum (ER) and Golgi were analyzed using 3D electron tomography of glands preserved by high-pressure freezing/freeze substitution methods.