Biotransformation and antioxidant enzymes of Limnoperna fortunei detect

VALESKA CONTARDO-JARA, LUCAS N.GALANTI, MARÍA V.AME, MAGDALENA V.MONFERRAN,

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ELSEVIER

Año: 2009 vol. 72 p. 1871 - 1880

0147-6513

The golden mussel  Limnoperna fortunei was used as a biomonitor of environmental pollution in the Suqu´ýa River basin around Córdoba City (Argentina). The sampling sites along the river were chosen according to the irincreasing levels of pollutants (e.g.heavy metals) as well as biological oxygen demand (BOD)and chemical oxygen demand (COD). A water quality index (WQI) was constructed from the  interaction of several normalized factors that affect the aquatic environment, such as the mentioned pollutants and physico-chemica lcharacteristics of the sampling sites. Activity changes of biotransfor- mation enzyme glutathione S-transferase (GST) and the antioxidant enzymes glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT), after exposure to pollutants, served as biomarkers. Membrane bound GST and antioxidant enzymes responded at the most polluted sampling site with in 1 day showing increased activities lasting for 4 days. Further sampling was restricted due to no survival of the animals. Antioxidant enzymes GPx, GR and CAT were sensitive responding to the different pollution scenarios, showing good correlation to the chemical characterization. Limnoperna fortunei was used as a biomonitor of environmental pollution in the Suqu´ýa River basin around Córdoba City (Argentina). The sampling sites along the river were chosen according to the irincreasing levels of pollutants (e.g.heavy metals) as well as biological oxygen demand (BOD)and chemical oxygen demand (COD). A water quality index (WQI) was constructed from the  interaction of several normalized factors that affect the aquatic environment, such as the mentioned pollutants and physico-chemica lcharacteristics of the sampling sites. Activity changes of biotransfor- mation enzyme glutathione S-transferase (GST) and the antioxidant enzymes glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT), after exposure to pollutants, served as biomarkers. Membrane bound GST and antioxidant enzymes responded at the most polluted sampling site with in 1 day showing increased activities lasting for 4 days. Further sampling was restricted due to no survival of the animals. Antioxidant enzymes GPx, GR and CAT were sensitive responding to the different pollution scenarios, showing good correlation to the chemical characterization.