INBA 12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
artículos
Título:
Improvement of biomass and cyclic lipopeptides production in Bacillus amyloliquefaciens MEP218 by modifying carbon and nitrogen sources and ratios of the culture media
Autor/es:
DANIELA MEDEOT; MAGDALENA BERTORELO-CUENCA; JUAN PABLO LIAUDAT; FLORENCIA ALVAREZ; MARÍA LAURA FLORES CÁCERES; EDGARDO JOFRÉ
Revista:
BIOLOGICAL CONTROL
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2017 vol. 115 p. 119 - 128
ISSN:
1049-9644
Resumen:
Bacillusamyloliquefaciens MEP218 is a native isolatewith a broad spectrum of antifungal activity against plant pathogenic fungi.The ability of strain MEP218 to antagonize phytopathogensis due to the production of cyclic lipopeptides (CLP). In this work, differentcarbon (C) and nitrogen (N) sources and C to N ratios were evaluatedin order to improve both, biomass and CLPproduction by strain MEP218. Amongthe C and N sources and C to N ratios tested, glucoseand NH4NO3 at the C to N ratio of 10 to 1 enhanced significantlybiomass and CLP yield. Moreover, CLP produced in this medium exhibited higherantibacterial activity against Xanthomonasaxonopodis pv. vesicatoria (Xav) thanthose obtained in the recommended medium for CLP production. Interestingly, CLP addition influenced the development of Xav biofilm on biotic and abioticsurfaces. A comparison of HPLC-chromatograms of CLP obtained in the optimizedmedium versus the obtained in the recommended medium showed a notable increment of surfactin in the CLP obtained in the optimizedmedium. Furthermore, two peaks with antibacterialactivity against Xav, identified by mass spectrometry analysis as fengycinsA and B, were detected in the CLP from the strain MEP218,grown in the optimized culture medium. Altogether, the results obtained in this work demonstratedthat changes in C and N sources and Cto N ratios affect the yield and type of CLP produced by B. amyloliquefaciens MEP218. The finding of fengycins withantibacterial activity constitutes, to our knowledge, the first report. CLPproduced by the strain MEP218 are potential candidates forcontrolling bacterial spot disease in tomato and pepper.
