Neonatal Hypoxia-Ischemia Reduces Ganglioside, Phospolipid And Cholesterol Contents In The Rat Hippocampus.

RAMIREZ, M. R., MURARO, F., ARTENI, N., LAVINSKY, D., NETTO, C. A. AND TRINDADE, V.M.T.

Neuroscience Research

Elsevier

Año: 2003 vol. 46 p. 339 - 347

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} p.MsoBodyTextIndent, li.MsoBodyTextIndent, div.MsoBodyTextIndent {margin-top:0cm; margin-right:2.45pt; margin-bottom:0cm; margin-left:0cm; margin-bottom:.0001pt; text-align:justify; text-indent:42.55pt; line-height:200%; mso-pagination:none; font-size:12.0pt; mso-bidi-font-size:10.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US; layout-grid-mode:line;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Hypoxia-ischemia is a common cause of neonatal brain damage producing serious impact on cerebral maturation. This report demonstrates that rats submitted to hypoxia-ischemia present a marked decrease in hippocampal ganglioside, phospholipid and cholesterol contents as from 7 days after the injury. Although chromatographic profiles of the different ganglioside species (GM1, GD1a, GD1b, and GT1b ) from the hippocampus of hypoxic-ischemic hippocampi groups (HI) were apparently unaffected, as compared to controls, there were quantitative absolute reductions in HI. The phospholipid patterns were altered in HI as from the 14th to the 30th  days after the injury, where phosphatidylcholine quantities were higher than phosphatidylethanolamine; additionally, the cardiolipin band was detected only in hippocampi of control adult rats.  In general the absolute quantities of phospholipids were lower in HI than in correspondent controls since 7th day after the injury. These effects were maintained and may reflect the late response caused by biochemical  cascades triggered by the neonatal hypoxic-ischemic episode.